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  Estonian Journal of Ecology

ISSN 1736-7549 (electronic)   ISSN 1736-602X (print)
An international scientific journal

Formerly: Proceedings of the Estonian Academy of Sciences: Biology, Ecology
(ISSN 1406-0914)
Published since 1952
 

Estonian Journal of Ecology

ISSN 1736-7549 (electronic)   ISSN 1736-602X (print)
An international scientific journal

Formerly: Proceedings of the Estonian Academy of Sciences: Biology, Ecology
(ISSN 1406-0914)
Published since 1952
 

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Toxins and other bioactive compounds produced by cyanobacteria in Lake Ladoga; pp. 100–110

(Full article in PDF format) doi: 10.3176/eco.2008.2.02


Authors

Ludmila Voloshko, Jiri Kopecky, Tatiana Safronova, Alla Pljusch, Nina Titova, Pavel Hrouzek, Valentina Drabkova

Abstract

Biologically active compounds were detected in water blooms on Lake Ladoga for the first time. Screening for cytotoxicity and for trypsin and acetylcholinesterase inhibitors revealed a degree of inhibitory activity by the water bloom biomass, which indicates the presence of cyano­bacterial toxins in Lake Ladoga. High performance liquid chromatography (HPLC) of extracts of selected samples from the lake demonstrated a rather high diversity of bioactive compounds including hepatotoxic cyclic peptides (microcystins), enzyme inhibitors (cytotoxins), and a series of unidentified substances. Thirteen toxins and protease inhibitors were identified by the HPLC. The toxins were most diverse in the most eutrophic southern part of the lake (up to 12 bioactive compounds). Chromato­grams showed that planktopeptin BL dominated in most of the samples investigated, except those from Svir Bay, where mcyst-LR prevailed. The results obtained indicate anthropogenic eutrophication of Lake Ladoga and a decrease in water quality. This lake is used as the only source of drinking water for the city of St. Petersburg.

Keywords

Lake Ladoga, cyanobacteria, toxin diversity.

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