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Estonian Journal of Ecology
Toxins and other bioactive compounds produced by cyanobacteria in Lake Ladoga; pp. 100–110
PDF | doi: 10.3176/eco.2008.2.02

Authors
Ludmila Voloshko, Jiri Kopecky, Tatiana Safronova, Alla Pljusch, Nina Titova, Pavel Hrouzek, Valentina Drabkova
Abstract
Biologically active compounds were detected in water blooms on Lake Ladoga for the first time. Screening for cytotoxicity and for trypsin and acetylcholinesterase inhibitors revealed a degree of inhibitory activity by the water bloom biomass, which indicates the presence of cyano­bacterial toxins in Lake Ladoga. High performance liquid chromatography (HPLC) of extracts of selected samples from the lake demonstrated a rather high diversity of bioactive compounds including hepatotoxic cyclic peptides (microcystins), enzyme inhibitors (cytotoxins), and a series of unidentified substances. Thirteen toxins and protease inhibitors were identified by the HPLC. The toxins were most diverse in the most eutrophic southern part of the lake (up to 12 bioactive compounds). Chromato­grams showed that planktopeptin BL dominated in most of the samples investigated, except those from Svir Bay, where mcyst-LR prevailed. The results obtained indicate anthropogenic eutrophication of Lake Ladoga and a decrease in water quality. This lake is used as the only source of drinking water for the city of St. Petersburg.
References

Banker, R. & Carmeli, S. 1999. Inhibitors of serine proteases from a waterbloom of the cyano­bacterium Microcystis sp. Tetrahedron, 55, 10835–10844.
doi:10.1016/S0040-4020(99)00597-9

Bateman, K. P., Thibault, P., Douglas, D. J. & White, R. L. 1995. Mass spectral analyses of micro­cystins from toxic cyanobacteria using on-line chromatographic and electrophoretic separations. J. Chromatogr., 712, 253–268.
doi:10.1016/0021-9673(95)00438-S

Bláha, L. & Maršálek, B. 2000. Methods for detection of cyanobacterial toxins – a review. Algol. Stud., 99, 1–22.

Botes, D. P., Wessels, P. L., Kruger, H., Runnegar, M. T. C., Santikarn, S., Smith, R. J., Barna, J. C. J. & Williams, D. H. 1985. Structural studies on cyanoginosins-LR, -YR, -YM, peptide toxins from Microcystis aeruginosa. J. Chem. Soc. Perkin Trans. I, 2747–2748.
doi:10.1039/p19850002747

Cannell, R. J. P., Kellam, S. J., Owsianka, A. M. & Walker, J. M. 1988. Results of a large-scale screen of microalgae for the production of protease inhibitors. Planta Med., 54, 10–14.
doi:10.1055/s-2006-962319

Carmichael, W. W. 1994. The toxins of cyanobacteria. Sci. Am. J., 270, 78–86.

Carmichael, W. W. 1997. The cyanotoxins. Adv. Bot. Res., 27, 211–256.
doi:10.1016/S0065-2296(08)60282-7

Codd, G. A., Lindsay, J., Young, F. M., Morrison, L. F. & Metcalf, J. S. 2005. From mass mortalities to management measures. In Harmful Cyanobacteria (Huisman, J., Matthijs, H. C. P. & Visser, P. M., eds), pp. 1–25. Springer, Netherlands.

Drabkova, V., Rumyantsev, V., Sergeeva, L. V. & Slepukhina, T. D. 1996. Ecological problems of Lake Ladoga, causes and solutions. Hydrobiologia, 322, 1–7.
doi:10.1007/BF00031798

Ellman, G. L., Courtney, K. O., Andrres, V. & Featherstone, R. M. 1961. A new and rapid colori­metric determination of acetylcholinesterase activity. Biochem. Pharmacol., 7, 88–95.
doi:10.1016/0006-2952(61)90145-9

Fujii, K., Sivonen, K., Adachi, K., Noguchi, K., Sano, H., Hirayama, K., Suzuki, M. & Harada, K. 1997. Comparative study of toxic and non-toxic cyanobacterial products: Novel peptides from toxic Nodularia spumigena AV1. Tetrahedron Lett., 38, 5525–5528.
doi:10.1016/S0040-4039(97)01192-1

Fujii, K., Sivonen, K., Naganawa, E. & Harada, K. 2000. Non-toxic peptides from toxic cyano­bacteria, Oscillatoria agardhii. Tetrahedron, 56, 725–733.
doi:10.1016/S0040-4020(99)01017-0

Grach-Pogrebinsky, O., Sedmak, B. & Carmeli, S. 2003. Protease inhibitors from a Slovenian Lake Bled toxic waterbloom of the cyanobacterium Planktothrix rubescens. Tetrahedron, 59, 8329–8336.
doi:10.1016/j.tet.2003.09.006

Gromov, В. V., Vepritsky, A. A., Mamkaeva, K. A. & Voloshko, L. N. 1996. A survey of cyano­bacterial blooms in Lake Ladoga and adjacent water bodies. Hydrobiologia, 322, 129–136.
doi:10.1007/BF00031820

Harada, K., Mayumi, T., Shimada, T., Fujii, K., Kondo, F., Park, H. & Watanabe, M. F. 2001. Co-production of microcystins and aeruginopeptins by natural cyanobacterial bloom. Environ. Toxicol., 16, 298–305.
doi:10.1002/tox.1036

Kuiper-Goodman, T., Falconer, I. & Fitzgerald, J. 1999. Human health aspects. In Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring, and Management (Chorus, I. & Bartram, J., eds), pp. 113–153. E & FN Spoon, London.

Lepistö, L., Rapala, J., Lyra, C., Berg, K., Erkomaa, K. & Isaakainen, J. 2005. Occurrence and toxicity of cyanobacterial blooms dominated by Anabaena lemmermannii P. Richter and Aphanizomenon spp. in boreal lakes in 2003. Algol. Stud., 117, 315–328.

Mahmood, N. A. & Carmichael, W. W. 1987. Anatoxin-a(s), an anticholinestease from the cyano­bacterium Anabaena flos-aquae NH-5. Toxicon, 25, 1221–1227.
doi:10.1016/0041-0101(87)90140-1

Meriluoto, J. A. O. & Eriksson, J. E. 1988. Rapid analysis of peptide toxins in cyanobacteria.
J.
Chromatogr., A810, 226–230.

Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol., 65, 55–63.

Namikoshi, M., Rinehart, K. L., Sakai, R., Sivonen, K. & Carmichael, W. W. 1990. Structures of three new cyclic hepatotoxins produced by the cyanobacterium (blue-green alga) Nostoc sp. strain 152. J. Org. Chem., 55, 6135–6139.
doi:10.1021/jo00312a019

Okano, T., Sano, T. & Kaya, K. 1999. Micropeptin T-20, a novel phosphate-containing cyclic depsipeptide from the cyanobacterium Microcystis aeruginosa. Tetrahedron Lett., 40, 2379–2382.
doi:10.1016/S0040-4039(99)00193-8

Rantala, A., Fewer, D. P., Hisbergues, M., Rouhiainen, L., Vaitomaa, J., Börner, T. & Sivonen, K. 2004. Phylogenetic evidence for the early evolution of microcystin synthesis. Proc. US Nat. Acad. Sci. (PNAS), 101, 568–573.

Sano, T & Kaya, K. 1995. Oscillamide Y, a chymotrypsin inhibitor from toxic Oscillatoria agardhii. Tetrahedron Lett., 36, 5933–5936.
doi:10.1016/0040-4039(95)01198-Q

Sivonen, K. 1996. Cyanobacterial toxins and toxin production. Phycologia, 35, 12–24.

Sivonen, K., Niemelä, S. I., Niemi, R. M., Lepistö, L., Luoma, T. H. & Rasanen, L. A. 1990. Toxic cyanobacteria (blue-green algae) in Finnish fresh and coastal waters. Hydrobiologia, 190, 267–275.
doi:10.1007/BF00008195

Sivonen, K., Namikoshi, M., Luukkainen, R., Färdig, M., Rouhiainen, L., Evans, W. R., Carmichael, W. W., Rinehart, K. L. & Niemelä, S. I. 1995. Variation of cyanobacterial hepatotoxins in Finland. In The Contaminants in the Nordic Ecosystem, Dynamics, Processes and Fate (Munawar, M. & Luotola, M., eds), pp. 163–169. Ecovision World Monograph Series. SPB Academic Publishing, Amsterdam.

Sivonen, K. & Jones, G. 1999. Cyanobacterial toxins. In Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring, and Management (Chorus, I. & Bartram, J., eds), pp. 41–111. E & FN Spoon, London.

Skulberg, O. M. 1993. Taxonomy of toxic cyanophyceae (Cyanobacteria). In Algal Toxins in Seafood and Drinking Water (Falconer, J. R., ed.), pp. 145–164. Academic Press Limited, London.

Skulberg, O. M. 1996. Toxins produced by cyanophytes in Norwegian inland waters – health and environment. In Chemical Data as a Basis of Geomedical Investigations (Låg, J., ed.),
pp. 179–216. The Norwegian Academy of Science and Letters, Oslo.

Sorokin, I., Velikoretskaya, I., Monina, T. & Rasumichina, V. 1995. The watershed of Lake Ladoga as a source of nutrients entering the lake. Publ. Karelian Inst., 112, 220–226. University of Joensuu.

WHO. 1998. Guidelines for Drinking Water Quality: Addendum to Volume 2: Health Criteria and Other Supporting Information. World Health Organization, Geneva.

Willen, N. & Mattsson, R. 1997. Water-blooming and toxin-producing cyanobacteria in Swedish fresh and brakish waters, 1981–1995. Hydrobiologia, 353, 181–192.
doi:10.1023/A:1003047019422

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